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TM phenotypes of tRNA modification mutants and their response to tRNAGln(UUG) overexpression. The indicated yeast strains were cultivated in YPD (a,c) or in YNB (b) 24 h with indicated concentrations of TM. Each experiment involved three biological replicates, and the resulting standard deviations are indicated on the bars. EV: empty vector; tQUUG: tRNAGln(UUG) expressing vector.
