. 2018 Oct 23;9(11):516. doi: 10.3390/genes9110516

Table 2.

Oligonucleotides used in this study.

Oligonucleotide	Sequence (5′–3′)	Target	References/Sources
Ire1_KO_Fwd	CATTAAAAAAACAGCATATCTGAGGAATTAATATTTTAGCACTTTGAAAACAGCTGAAGCTTCGTACGC	pUG27, pUG72 /IRE1	This study
Ire1_KO_Rev	TAACATTAATGCAATAATCAACCAAGAAGAAGCAGAGGGGCATGAACATGGCATAGGCCACTAGTGGATCTG	pUG27, pUG72 /IRE1	This study
Ire1_KO+_Fwd	CTTCGGGCAATACCTTCGACT	IRE1	This study
Ire1_KO+_Rev	CAACCAAGAAGAAGCAGAGGG	IRE1	This study
KO_NCS2_FW	TGCTATTGTCCATCCCTATCCTAGTTTTAAAAATATAATTCTATCAAGTTCAGCTGAAGCTTCGTACGC	pUG27 /NCS2	This study
KO_NCS2_RV	TAAATAAATAAATACATAACCATTGGAATAGCGAAGCCTTTGACATTTCAGCATAGGCCACTAGTGGATCTG	pUG27 /NCS2	This study
N_NCS2_FW	ACCGATGAGATGAGTGAGAC	NCS2	This study
pUG27/SpHIS rev	GTCCAAAGCGATGGCAACGC	SpHIS5	This study
HAC1_qPCR_Fwd	GACGACGCTACCTGCCG	HAC1ⁱ	This study
HAC1_qPCR_Rev	ACTGCGCTTCTGGATTACG	HAC1ⁱ	This study
qPCR_ACT1_FW	TTCCAGCCTTCTACGTTTCC	ACT1	[16]
qPCR_ACT1_RV	AATCTCTACCGGCCAAATCG	ACT1	[16]
HAC1F	CTGGCTGACCACGAAGACGC	HAC1^u/i	[34]
HAC1R	TTGTCTTCATGAAGTGATGA	HAC1^u/i	[34]