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. Author manuscript; available in PMC: 2018 Dec 2.
Published in final edited form as: Cell Rep. 2018 Oct 23;25(4):947–958.e4. doi: 10.1016/j.celrep.2018.09.083

Figure 1. Effects of GFAP Mutations on Astrocyte Differentiation.

Figure 1.

(A) Cartoon depicting relative locations of AxD mutations on a GFAP monomer along with an introduction of cell lines.

(B) Sanger sequencing of patient-derived IPSCs and corrected isogenic lines.

(C) Quantification of GFAP+ cells at 3 months (gray) and 6 months (black) during astrocyte differentiation (n = 4).

(D) TaqMan SNP results showing relative RNA detection for each specific probe (n = 3).

(E) Expression of astrocyte-associated markers by qPCR (n = 4).

(F) Immunocytochemistry of 6 month astrocytes for GFAP, S100B, and Sox9. Scale bars, 100 μm.

Data are represented as mean ± SD.