(A) Cartoon depicting relative locations of AxD mutations on a GFAP
monomer along with an introduction of cell lines.
(B) Sanger sequencing of patient-derived IPSCs and corrected isogenic
lines.
(C) Quantification of GFAP+ cells at 3 months (gray) and 6 months
(black) during astrocyte differentiation (n = 4).
(D) TaqMan SNP results showing relative RNA detection for each specific
probe (n = 3).
(E) Expression of astrocyte-associated markers by qPCR (n = 4).
(F) Immunocytochemistry of 6 month astrocytes for GFAP, S100B, and Sox9.
Scale bars, 100 μm.
Data are represented as mean ± SD.