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. Author manuscript; available in PMC: 2018 Dec 2.
Published in final edited form as: Cell Rep. 2018 Oct 23;25(4):947–958.e4. doi: 10.1016/j.celrep.2018.09.083

Figure 2. GFAP Expression and Aggregation in Differentiated Astrocytes.

Figure 2.

(A) Immunocytochemistry for GFAP showing perinuclear GFAP+ puncta (arrowheads). Scale bar, 10 μm.

(B) STED microscopy images on astrocytes stained for GFAP (red) and αB-crystallin (green). Scale bar, 2 μm.

(C) Electron micrographs of aggregated GFAP (arrowheads). Scale bar, 800 nm.

(D) Quantification of cells exhibiting punctate GFAP signal over total GFAP population (n = 4).

(E) Western blot from 6 month astrocyte lysates probed for GFAP, xαB-crystallin, and actin.

(F) Densitometry analysis for GFAP (n = 3).

(G) Densitometry analysis for αB-crystallin (n = 3).

*p ≤ 0.05. Data are represented as mean ± SD.