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. Author manuscript; available in PMC: 2018 Dec 2.
Published in final edited form as: Cell Rep. 2018 Oct 23;25(4):947–958.e4. doi: 10.1016/j.celrep.2018.09.083

Figure 6. ATP Secretion Deficits in AxD Astrocytes.

Figure 6.

(A) Still images over the duration (2 min) of mechanical stimulation on wild-type 6 month astrocytes ± apyrase with accompanying post-experiment GFAP stains.

(B) Average duration of increased calcium signal above threshold ± apyrase (n = 10).

(C) Average percentage of field traveled by Ca2+ wave ± apyrase (n = 10).

(D) Maximum fluorescence intensity achieved after bath application of 100 μm ATP ± apyrase (n = 10).

(E) Average results of ATP production via seahorse metabolic profiling (n = 3).

(F) Average ATP concentration detected in media ± OAG treatment (n = 3).

(G) Average ATP concentration remaining in cells ± OAG treatment (n = 3).

**p = 0.001–0.01 and ***p < 0.001. Scale bars, 100 μm. Data are represented as ± SEM.