(A) Still images over the duration (2 min) of mechanical stimulation on
wild-type 6 month astrocytes ± apyrase with accompanying post-experiment
GFAP stains.
(B) Average duration of increased calcium signal above threshold
± apyrase (n = 10).
(C) Average percentage of field traveled by Ca2+ wave
± apyrase (n = 10).
(D) Maximum fluorescence intensity achieved after bath application of
100 μm ATP ± apyrase (n = 10).
(E) Average results of ATP production via seahorse metabolic profiling
(n = 3).
(F) Average ATP concentration detected in media ± OAG treatment
(n = 3).
(G) Average ATP concentration remaining in cells ± OAG treatment
(n = 3).
**p = 0.001–0.01 and ***p < 0.001. Scale bars, 100
μm. Data are represented as ± SEM.