Antibodies |
Mouse anti-αB Crystallin |
Abcam |
Cat# ab13496, RRID: AB_300400 |
Rabbit anti-Connexin43 |
Abcam |
Cat# ab11370, RRID: AB_297976 |
Rabbit anti-ERp57 |
Proteintech |
Cat# 15967-1-AP, RRID: AB_2236784 |
Rabbit anti-GFAP |
DAKO |
Cat# Z0334, RRID: AB_10013382 |
Mouse anti-GFAP |
Millipore |
Cat# IF03L, RRID: AB_2294571 |
Rabbit anti-GM130 |
Cell Signaling Technologies |
Cat# 2296S, RRID: AB_10695240 |
Mouse anti-LAMP2 |
Novus |
Cat# NBP2-22217, RRID: AB_2722697 |
Mouse anti-S100B |
Abcam |
Cat# ab66028, RRID: AB_1142710 |
Goat anti-Sox9 |
R&D Systems |
Cat# AF3075, RRID: AB_2194160 |
Rat anti-tubulin |
Abcam |
Cat# ab6160, RRID: AB_305328 |
Alexa Fluor 546 donkey anti-rabbit IgG
(H+L) |
Molecular Probes |
Cat# A10040 |
Alexa Fluor 488 donkey anti-mouse IgG
(H+L) |
Molecular Probes |
Cat# A21202 |
Alexa Fluor 488 donkey anti-goat IgG
(H+L) |
Molecular Probes |
Cat# A11055 |
Alexa Fluor 647 Donkey Anti-rat IgG (H+L) |
Abcam |
Cat# ab150155 |
Alexa Fluor
647 Donkey Anti-mouse IgG (H+L) |
Molecular
Probes |
Cat#
A-31571 |
Critical Commercial Assays |
ATPlite Luminescence Assay |
Perkin-Elmer |
Cat# 6016941 |
RNeasy mini kit |
QIAGEN |
Cat# 74106 |
Seahorse XF Cell Mito Stress Test Kit |
Seahorse Biosciences |
Cat# 103015-100 |
Seahorse XF
Glycolytic Rate Assay Kit |
Seahorse
Biosciences |
Cat#
103344-100 |
Experimental Models: Cell Lines |
H1 human embryonic stem cells |
WiCell Research Institute |
WA01 |
AxD patient fibroblasts (C88) |
Waisman center iPSC core |
WC-01-01-AL-AM |
AxD patient fibroblasts (W416) |
Waisman center iPSC core |
WC-14-01-AL-AM |
R236H/+
primary astrocytes |
Generously
provided by Dr. Albee Messing |
N/A |
Software and Algorithms |
Enrichr |
Ma’ayan lab |
http://amp.pharm.mssm.edu/Enrichr/ |
Harmony |
PerkinElmer |
http://www.perkinelmer.com/product/harmony-4-6-office-hh17000001 |
ImageJ |
National Institutes of Health |
https://imagej.nih.gov/ij/ |
Morpheus |
Broad Institute |
https://software.broadinstitute.org/morpheus/ |
NIS Elements |
Nikon |
https://www.nikoninstruments.com/Products/Software |
Prism |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
R-Studio |
RStudio |
https://www.rstudio.com |