KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-αB Crystallin | Abcam | Cat# ab13496, RRID: AB_300400 |
| Rabbit anti-Connexin43 | Abcam | Cat# ab11370, RRID: AB_297976 |
| Rabbit anti-ERp57 | Proteintech | Cat# 15967-1-AP, RRID: AB_2236784 |
| Rabbit anti-GFAP | DAKO | Cat# Z0334, RRID: AB_10013382 |
| Mouse anti-GFAP | Millipore | Cat# IF03L, RRID: AB_2294571 |
| Rabbit anti-GM130 | Cell Signaling Technologies | Cat# 2296S, RRID: AB_10695240 |
| Mouse anti-LAMP2 | Novus | Cat# NBP2-22217, RRID: AB_2722697 |
| Mouse anti-S100B | Abcam | Cat# ab66028, RRID: AB_1142710 |
| Goat anti-Sox9 | R&D Systems | Cat# AF3075, RRID: AB_2194160 |
| Rat anti-tubulin | Abcam | Cat# ab6160, RRID: AB_305328 |
| Alexa Fluor 546 donkey anti-rabbit IgG (H+L) | Molecular Probes | Cat# A10040 |
| Alexa Fluor 488 donkey anti-mouse IgG (H+L) | Molecular Probes | Cat# A21202 |
| Alexa Fluor 488 donkey anti-goat IgG (H+L) | Molecular Probes | Cat# A11055 |
| Alexa Fluor 647 Donkey Anti-rat IgG (H+L) | Abcam | Cat# ab150155 |
| Alexa Fluor 647 Donkey Anti-mouse IgG (H+L) | Molecular Probes | Cat# A-31571 |
| Critical Commercial Assays | ||
| ATPlite Luminescence Assay | Perkin-Elmer | Cat# 6016941 |
| RNeasy mini kit | QIAGEN | Cat# 74106 |
| Seahorse XF Cell Mito Stress Test Kit | Seahorse Biosciences | Cat# 103015-100 |
| Seahorse XF Glycolytic Rate Assay Kit | Seahorse Biosciences | Cat# 103344-100 |
| Experimental Models: Cell Lines | ||
| H1 human embryonic stem cells | WiCell Research Institute | WA01 |
| AxD patient fibroblasts (C88) | Waisman center iPSC core | WC-01-01-AL-AM |
| AxD patient fibroblasts (W416) | Waisman center iPSC core | WC-14-01-AL-AM |
| R236H/+ primary astrocytes | Generously provided by Dr. Albee Messing | N/A |
| Software and Algorithms | ||
| Enrichr | Ma’ayan lab | http://amp.pharm.mssm.edu/Enrichr/ |
| Harmony | PerkinElmer | http://www.perkinelmer.com/product/harmony-4-6-office-hh17000001 |
| ImageJ | National Institutes of Health | https://imagej.nih.gov/ij/ |
| Morpheus | Broad Institute | https://software.broadinstitute.org/morpheus/ |
| NIS Elements | Nikon | https://www.nikoninstruments.com/Products/Software |
| Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| R-Studio | RStudio | https://www.rstudio.com |