Figure 2.
Coomassie blue stained SDS gel (4–30% gradient polyacrylamide) for samples purified on Hupresin. Samples had been boiled in the presence of dithiothreitol to reduce disulfide bonds. Lanes 1–6 and lanes 9–11, truncated rHuBChE with specific activities of 630, 596, and 531 u/mg. Lane 8, plasma-derived HuBChE purified from frozen Cohn fraction IV-4.