Stromal fibroblasts from matched EM, Cx and ECx were pretreated for 48hr with E2 (5×10−8M) and subsequently retreated with poly (I:C), E2, or a combination of both for a further 24hr. Conditioned media was recovered and analyzed for SDF-1α (A), MCP-1 (B), CCL20 (C), IL-6 (D), IL-8 (E), RANTES (F) and IFNβ (G) by ELISA (n=4). Bars and horizontal lines represent the mean cytokine concentration +/− SEM respectively, for independent experiments using matched EM, Cx and ECx from 4 different donors.