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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Am J Reprod Immunol. 2018 Sep 17;80(5):e13042. doi: 10.1111/aji.13042

Figure 8: E2 stimulates SDF-1α secretion by endometrial fibroblasts.

Figure 8:

EM stromal fibroblasts were pretreated for 48hr with E2 (5×10−8M) and subsequently retreated with E2 for a further 24hr. Conditioned media was recovered and analyzed for SDF-1α by ELISA (A) (n=4). Cultured EM stromal fibroblasts were pretreated for 1 hr using the ERα antagonist Raloxifene (Rx) (5×10−6M), followed by addition of E2 (5×10−8M) for a further 48hr during which Rx was maintained in the culture media. Conditioned media was recovered and SDF-1α concentration determined by ELISA (B). Data shown in (A) is the mean +/− SEM of individual experiments from 4 individual donors while (B) shows the mean +/− SEM of a single donor that is representative of independent experiments from 3 individual donors. ** P<0.01, ***P<0.001. Bars and horizontal lines in both panels represent the mean +/− the SEM respectively.