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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Glia. 2018 Oct 30;66(11):2427–2437. doi: 10.1002/glia.23482

Figure 1.

Figure 1

Astrocyte restricted depletion of Parkin impairs neuronal survival in cocultures. (a) Quantitation of LDH released in response to 6-OHDA and MPP+ (a toxic metabolite of MPTP) from SHSY5Y cells cocultured with WT or parkin KO primary astrocytes. (b) Quantitation of LDH released from SHSY5Y cells transduced with control (cont) or parkin shRNA lentivirus and exposed to indicated drugs. (c) Quantitation of LDH released in response to ER stress induced by thapsigargin (TG) or in response to TG and lipopolysaccharide (LPS) exposure in SHSY5Y cells cocultured with either WT or parkin KO primary astrocytes. (d) Representative immunoblot showing levels of total and cleaved PARP levels in SHSY5Y cells extracts in the same coculture conditions described in panel 1c. (e) Quantitation of LDH released in response to increasing doses of TG from primary cortical neurons cocultured with either WT or parkin KO astrocytes. (f) A representative immunoblot showing the TG dose-mediated effects on total and cleaved PARP in the primary neurons cocultured with either WT or parkin KO astrocytes. Statistical difference was assessed by student’s t-test. *p<0.05 and **p<0.01, compared to the corresponding controls. n.s = not significant. All experiments were repeated ≥ 3 times.