(A) VISTA comparison of the Fgf10 locus in human vs mouse (upper) and human vs mouse vs chick vs frog (lower). Peaks with orange color indicate conserved non-coding sequences. The Fgf10 gene and exons are indicated with a bar and boxes at the top. Previously characterized sequences are shown with blue columns with their names at the top. The six sequences in intron 1 used in this study are indicated by light gray columns with their names on the top.
(B) Schematic presentation of −0.34k promoter-luciferase vector (upper) and a construct with a tested cis-element.
(C) Comparison of activities of −0.34k promoter construct and the six constructs with indicated cis-elements. Luciferase activities are shown as mean ± S.D.