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Estimates of Michaelis–Menten Model Parameters for Immobilized and Free Calf Intestinal Alkaline Phosphatase
| conventional estimates |
bootstrap estimates |
|||||
|---|---|---|---|---|---|---|
| condition | Km (μM) | kcat(s−1)a | Vmax (nM/s) | Km (μM) | kcat(s−1)a | Vmax (nM/s) |
| immobilized | 9.3 ± 1.1 | 1.2 ± 0.2 | 4.1 ± 0.2 | 9.3 ± 0.3 | 1.2 ± 0.1 | 4.1 ± 0.05 |
| in-solution | 5.3 ± 0.7 | 230.2 ± 12.5 | 106.8 ± 3.5 | 5.3 ± 0.4 | 229.7 ± 11.5 | 106.6 ± 2.7 |
[CIAPimmobilizedl = 3.4 ± 0.4 nM. [CIAPin-solution] = 0.464 ± 0.02 nM.
