Reduction of T cells was found in the periphery in 8-week-old Klotho-deficient mice that can be prevented by deprivation of vitamin D.
(A) Frequency and (B) cellularity of CD4T and CD8T cells in the spleen from Kl/Kl mice and littermate controls at 8 weeks of age. (C) Frequency and (D) cellularity of CD44hi memory-like T cells subpopulations in the spleen from Kl/Kl mice and littermate controls at 8 weeks of age. (E) Frequency and (F) cellularity of CD4T and CD8T cells in the spleen from 8-wk-old Kl/Kl mice and littermate controls fed with vitamin D-deprived diet. Each symbol in the graphs represents an individual mouse (n ≥ 4); small horizontal lines indicate the group mean (± s.d.). ns, not significant (P ≥ 0.05); *, P < 0.05.