FIG 4.

Med14 depletion impairs transcription of sn/snoRNA genes. (A) Western blot showing the kinetics of Med14-AID depletion. A histone H3 blot is shown as a loading control. (B) Spot assays analyzing the growth of parental and Med14-AID strains on YPD containing DMSO or 500 μM 3-IAA. (C) Levels of total and newly synthesized RNA for transcripts produced by all three RNAPs, as measured by 4tU labeling and qRT-PCR in DMSO- and 3-IAA-treated Med14-AID cells. Error bars represent the average plus SEM for two biological replicates.