Figure 3.

mir‐382‐5p overexpression in CB CD34+ cells reduces SOD activity, and increases ROS production and oxidative DNA damage. (A) Effect of miR‐382‐5p overexpression on SOD activity (U·mL⁻¹) determined 24 h after the last nucleofection. (B) The intracellular level of ROS in miR‐382‐5p and mimic‐Neg was measured using DCF staining by flow cytometric analysis 24 h after the last nucleofection. (C) Representative flow cytometry dot plots showing the side scatter (SSC) versus DCF fluorescence distribution in miR‐382‐5p‐overexpressing cells and mimic‐Neg sample at 24 h after the last nucleofection. (D) The level of 8‐OH‐dG in miR‐382‐5p and mimic‐Neg was measured at 24 h after the last nucleofection. The results are representative of six independent experiments and are expressed as the mean ± SEM. Two‐tailed Student's t‐test: **P < .01 versus mimic‐Neg; *P < .05 versus mimic‐Neg.