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. 2018 Oct 30;12(12):2147–2164. doi: 10.1002/1878-0261.12393

Figure 1.

Figure 1

The efficacy of gemcitabine chemotherapy is improved in PC cells by PVT1 inhibition. (A,B) Expression of MYC (A) and PVT1 (B) in PC cells was determined by qRT‐PCR. Data were normalized to GAPDH. (C) Cell growth was analyzed in BxPC‐3 (left) and PANC‐1 (right) cells with gemcitabine treatment by using a CCK‐8 assay at 12 h intervals up to 72 h. (D,E) Apoptosis assays were used to measure programmed cell death in BxPC‐3 (D) and PANC‐1 (E) cells upon PVT1 inhibition and gemcitabine treatment. Normalization of the apoptotic cells is shown on the right in (D) and (E). (F,G) Cell cycle analyses were performed in BxPC‐3 (upper) and PANC‐1 (lower) cells (F) and normalization of cell numbers at G1‐, S‐ and G2/M‐phase is shown in (G). *P < 0.05, **P < 0.01, ***P < 0.001. Mean ± SD values were calculated from triplicate samples. P values were based on Student's t test unless otherwise indicated.