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. 2018 Oct 30;12(12):2147–2164. doi: 10.1002/1878-0261.12393

Figure 4.

Figure 4

Gemcitabine suppresses PC cell growth by regulating miR‐1207‐induced signaling. (A,B) Expression of PVT1 (A) and primary miR‐1207 (Pri‐1207) (B) was evaluated by qRT‐PCR analysis in BxPC‐3 and PANC‐1 cells treated with gemcitabine. GAPDH served as a loading control. (C) Expression of miR‐1207‐5p and miR‐1207‐3p was assessed in BxPC‐3 and PANC‐1 cells with gemcitabine treatment. U6 snRNA was used as a loading control. (D,E) Expression of miR‐1207 pair was evaluated in BxPC‐3 (D) and PANC‐1 (E) cells in which rescue assays were conducted. (F,G) The protein levels of SRC (F) and RhoA (G) were assessed in BxPC‐3 and PANC‐1 cells in which rescue assays were conducted. (H,I) Apoptosis assays were used in the rescue experiments that were conducted in BxPC‐3 (H) and PANC‐1 (I) cells. The percentage of apoptotic cells is shown (bottom). (J,K) Cell cycle analyses were performed in the rescue experiments that were conducted in BxPC‐3 (J) and PANC‐1 (K) cells. The relative cell numbers at the G1‐, S‐ and G2/M‐phase are shown (bottom ). *P < 0.05, **P < 0.01, ***P < 0.001. Mean ± SD values were calculated from triplicate samples. P values were based on Student's t test unless otherwise indicated.