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. 2018 Nov 5;12(12):2191–2208. doi: 10.1002/1878-0261.12395

Figure 2.

Figure 2

AXL attenuates epirubicin‐induced apoptosis. (A) OE33/pcDNA4 and OE33/AXL stable cells were treated with vehicle or epirubicin (0.5 μm) for 24 h. Apoptosis was determined by Annexin‐V/DAPI staining and FACS analysis. (B) Immunoblot analysis of AXL, caspase‐3, and PARP proteins in OE33/pcDNA4 and OE33/AXL cells after treatment with vehicle or epirubicin as described in panel A. (C) Apoptosis in FLO‐1/control shRNA and FLO‐1/AXL shRNA cells after treatment with vehicle or epirubicin (1 μm) for 24 h was assessed as in panel A. (D) Western blot analysis of AXL, caspase‐3, and PARP proteins in FLO‐1/control shRNA and FLO‐1/AXL shRNA cells after treatment with vehicle or Epirubicin as in panel C. Gel loading was normalized for equal β‐actin. Results from at least three independent experiments are represented as mean ± SEM. Statistical significance was evaluated by Student's t‐test.