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. 2018 Nov 5;12(12):2191–2208. doi: 10.1002/1878-0261.12395

Figure 4.

Figure 4

AXL upregulates expression and activity of c‐MYC in EAC cells. Western blot analysis of AXL and c‐MYC proteins in OE33/pcDNA4 and OE33/AXL cells (A) and in FLO‐1/Control shRNA and FLO‐1/AXL shRNA cells (B). Gel loading was normalized for equal β‐actin. Real‐time quantitative PCR analysis of c‐MYC and AXL in OE33/pcDNA4 and OE33/AXL cells (C, E), and in FLO‐1/Control shRNA and FLO‐1/AXL shRNA cells (D, F). To measure the c‐MYC transcriptional activity, OE33/pcDNA4, OE33/AXL, FLO‐1/Control shRNA, and FLO‐1/AXL shRNA cells were transfected with 4xEMS‐Luc reporter and β‐galactosidase plasmids (G, H). Data from three independent experiments are represented as mean ± SEM. Statistical significance was evaluated by Student's t‐test.