Figure 4.

RAB31 promotes GC progression through activation of the GLI1 pathway in vivo. (A) Western blotting was performed to evaluate the overexpression of RAB31 after transfection of plasmids into MKN45 cells. (B–F) AGS and MKN45 cells with enhanced RAB31 expression and vector control were transfected simultaneously with GLI1 siRNA and siNC, respectively. (B,C) The viability of AGS and MKN45 cells was determined by the CCK8 assay. (D) Representative images of colony formation assays are shown on the left; the average colony formation from three experiments is shown by the histogram on the right (P < 0.01). (E,F) Cell cycle and apoptosis were assessed by flow cytometry. Representative images are on the left and the results of triplicate assays are shown on the right. Results are shown as the mean ± SD (^*P < 0.05, ^**P < 0.01, and ^***P < 0.001).