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. 2018 Nov 26;8:554. doi: 10.3389/fonc.2018.00554

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Copyright © 2018 Tang, Liang, Yang, Lin, Wu, Chen, Zhang, Gao and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-30c-2-3p is involved in the RAB31/GLI1 pathway and regulates GC cell growth and apoptosis via binding to the 3′-UTR of RAB31. (A) Luciferase reporter assay was performed using MKN45 cells to determine if miR-30c-2-3p binds to the 3′-UTR of RAB31. (B,C) RAB31, GLI1, cyclin D1, BAX, and Bcl-2 expression was detected by Western blotting in AGS and MKN45 cells after transfection with miR-30c-2-3p mimics or the inhibitor; GAPDH was used as the control. (D,E) Cell viability was assessed in AGS and MKN45 cells transfected with the miR-30c-2-3p inhibitor and RAB31 siRNA. (F,G) Western blotting was conducted to investigate the levels of RAB31, GLI1, and its downstream proteins in AGS and MKN45 cells co-transfected with the miR-30c-2-3p inhibitor.