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Semi-quantitative PCR analysis of the MAwuPI expression pattern in Magnolia wufengensis. For this assay, flower buds, and juvenile leaves were collected from M. wufengensis growing in the secondary natural forest. The flower buds were separated into outer-tepals, inner-tepals, stamens, and carpels, and frozen immediately in liquid nitrogen. Three biological replicates were performed for each sample. le, leaf; te, tepal; st, stamen; ca, carple; actin as internal control.
