Figure 7.

Loss of gap junction coupling affects hypothalamic cell proliferation. Intraperitoneal injection of bromodeoxyuridine (BrdU) was performed as described in (A). (B) Number of BrdU-positive cells in the parenchyma was quantified in three dKO (13 slices) and four wild type mice (23 slices) and the results were normalized to the area analyzed (μm²), using the ROI manager of ImageJ software. Statistical analysis was performed using the non-parametric Mann Whitney one tail test, *P < 0.05. Representative immunohistochemistry using anti-BrdU (green) and anti-GFAP (magenta) antibodies in frontal sections of WT (C,C′) and dKO (D,D′) animals. Boxes in (C′,D′) indicate co-localization of anti-BrdU with nuclei staining (Hoechst). Arrowheads indicate BrdU staining close to the 3V, while arrows indicate BrdU-positive cells distal to the 3V. 3V, third ventricle. Scale bar (C,C′,D,D′): 50 μm, Scale bar (Hoechst box): 10 μm.