Figure 1.

RNase A treatment increases numbers of NPCs in dissociated neuronal cultures. Mixed mouse cortex and hippocampus cultures were treated with 100 μg/ml BSA or Invitrogen RNase A at 1 DIV and grown for 3 days. (A) Representative images of immunostaining with Nestin, an NPC marker, are shown. Counter-staining with DAPI was performed to label cell nuclei. The number of DAPI⁺ cells represents the total cell number. (B) Quantifications, including the number of total DAPI⁺ cells, the number of Nestin⁺ cells and the percentage of Nestin⁺ cells in total DAPI⁺ cells. Mean and SD of three independent experiments are shown. Scale bars, 50 μm. Statistical analyses were performed using unpaired t-tests. ^**P < 0.01; ^***P < 0.001.