FIG 1.

Amentoflavone inhibits SLY pore-forming activity. (A) Chemical structure of amentoflavone. (B) Growth curves of SS2 cultured in Todd-Hewitt broth containing 2% yeast extract and 5% FBS and treated with different concentrations of amentoflavone. (C) Hemolytic activity of supernatants from SS2 and amentoflavone coculture system. (D) Western blot of SLY expression in the culture supernatants with or without amentoflavone. (E) Inhibitory effect of amentoflavone on hemolytic activity of purified SLY (100 ng/ml). The data shown are representative of three independent experiments. *, P < 0.05; **, P < 0.01 versus 0 μg/ml.