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Amentoflavone inhibited SS2-mediated cytokine production within J774 cells. Amentoflavone inhibited the secretion of TNF-α (A), IL-1β (B), and IL-6 (C). Cells were incubated with SS2 (MOI = 10:1) and various concentrations of amentoflavone for 6 h. TNF-α, IL-1β, and IL-6 concentrations in culture medium were assayed by ELISA. ND, nondetectable; *, P < 0.05; **, P < 0.01 versus SS2 alone.
