TABLE 3.
Plasmids utilized for this study
| Backbone | Cassette shorthandc | Cloning site contentsd |
|---|---|---|
| pCDFDuet1a | –/– | NA/NA |
| acI-CrtEBI/− | acI-crtE, acI-crtB, acI-crtI/NA | |
| acI-CrtEBI/Pa-CrtY | acI-crtE, acI-crtB, acI-crtI/Pa-crtY | |
| Pa-CrtEBI/− | Pa-crtE, Pa-crtB, Pa-crtI/NA | |
| Pa-CrtEBIY/− | Pa-crtE, Pa-crtB, Pa-crtI, Pa-crtY/NA | |
| Pa-CrtEBIY/acI-Blh | Pa-crtE, Pa-crtB, Pa-crtI, Pa-crtY/acI-blh | |
| pET21b+b | – | NA |
| acI-ActRL06 | acI-actRL06 |
a
Plasmid contains two cloning sites with contents separated by a slash.
b
Plasmid contains one cloning site in-frame with a C-terminal hexahistidine tag.
c
–, empty.
d
NA, not applicable because the cloning site is unmodified.