TABLE 4.
Primers for this studya
| Category | Name | Sequence (5′–3′) |
|---|---|---|
| Genomic | F_EIB | ATGCAGAGCGCATCAGAGGT |
| R_EIB | TTAGCCAATTCGTGCTGCAA | |
| F_YcYdBlh | TTAGTGTCTTTGCAGCCCT | |
| R_YcYdBlh | CTAACGAAGGTGCGATTTAT | |
| CrtEBI/− and CrtEBI/PaY | F_E_NcoI | aaataattttgtttaactttaataaggagatataccatggATGCAGAGCGCATCAGAGGT |
| R_E_AflII | tgtacaatacgattactttctgttcgacttaagTTAGTAGCTCCTACGGATTGCTGTCTC | |
| F_B_Erbs | agcaatccgtaggagctactaacaagaaggagatatacttATGGATGCAGAACTTGCAGC | |
| R_B_EcoAfl | cgtgtacaatacgattactttctgttcgacttaagaattcttaGCCAATTCGTGCTGCAA | |
| F_E/−_BEcoAfl | attgcagcacgaattggctaagaattcTTAAGTCGAACAGAAAGTAATCGTATTGTACAC | |
| R_E/−_ErbsB | caagttctgcatccataagtatatctccttcttGTTAGTAGCTCCTACGGATTGCTGTCT | |
| F_I_Brbs | gcagcacgaattggctaagaagaaggagatatacttATGACAAGAAAAGTTAAGGGACCC | |
| R_I_EcoRIAflII | tgtacaatacgattactttctgttcgacttaagaattttaCTTGACCGGACCCACAATAC | |
| F_EB/−_IEcoAfl | cgtattgtgggtccggtcaagtaagAATTCTTAAGTCGAACAGAAAGTAATCGTATTGTA | |
| R_EB/−_BrbsI | ggtcccttaacttttcttgtcataagtatatctccttcttCTTAGCCAATTCGTGCTGCA | |
| F_PaY_NdeI | tcttagtatattagttaagtataagaaggagatatacatATGCAACCGCATTATGATCTG | |
| R_PaY_AvrII | atgctagttattgctcagcggtggcagcagcctaggTTAACGATGAGTCGTCATAATGGC | |
| PaCrtEBIY/−, PaCrtEBIY/Blh, and PaCrt EBI/− | F_PaEBIY_NcoI | ataattttgtttaactttaagaaggagatataccatggATGACGGTCTGCGCAAAAAAAC |
| R_PaEBIY_AfIII | acaatacgattactttctgttcgacttaagttatTTAACGATGAGTCGTCATAATGGCTTG | |
| F_PaI_H246H | GCCAGAGTCAGCCAcATGGAAACGACAGGAAAC | |
| R_PaI_H246H | GTTTCCTGTCGTTTCCATgTGGCTGACTCTGGC | |
| F_PaEBIY_PaY | TAATAACTTAAGTCGAACAGAAAGTAATCGTATTGTAC | |
| R_PaEBIY_PaI | agatatacttaagTTATTATATCAGATCCTCCAGCATCAAAC | |
| F_blh_NdeI | tattagttaagtataagaaggagatatacatATGGAGATGGCAAAGTTAAAGACATTTTC | |
| R_blh_AvrII | tattgctcagcggtggcagcagcctaggttaCTAACGAAGGTGCGATTTATTCTTGAGAG | |
| ActR | F_ActRB_NdeI | aaataattttgtttaactttaagaaggagatatacatATGTCGAGCACCATCG |
| R_ActRB_XhoI | atctcagtggtggtggtggtggtgctcgagATGGTCGTCTTTCATGCC |
a
Annealing regions are indicated with capital letters, while mismatches or overhangs are lowercase.