FIG 5.

Rarefaction curves of the number of OTUs, the Chao1 estimator, and the Shannon index using OTU similarity thresholds of 97% (A) and 90% (B) for the amoA gene among different comammox clades, AOB, and AOA, based on a combination of 1,900 randomly selected reads from each of the 12 different environmental samples from which comammox, AOB, and AOA amoA genes were efficiently amplified. For diversity index comparison of amoA genes of comammox, AOB, and AOA for each sample, please see Table S6.