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. 2018 Nov 18;21(1):27–34. doi: 10.22074/cellj.2019.5717

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Fig.4 — Effect of amentoflavone on the expression of proteins related to p53 signaling pathway. A. The images of p53 (FITC) and p-p21 (CY3) immunofluorescence-stained A549 cells were shown by the green and red color, respectively. Arrows show p53 and p-p21 have been localized to the cell cytosol or nuclear (scale bar: 100 µm), B. The effect of amentoflavone on protein expressions of p-p21, p53, ac-p53, and p-p53 was analyzed by western blot, and C. The level of protein expressions was quantified by Multi Gauge V3.0 software.