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. 2018 Nov 9;115(48):E11331–E11340. doi: 10.1073/pnas.1720209115

Fig. 1.

Fig. 1.

Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. (A) Tyrosine phosphatase activity assay of immunoprecipitated Shp1 on ex vivo thymocytes from Themis+/+ and Themis−/− mice with and without stimulation using biotinylated antibody cross-linking with streptavidin. (B) Tyrosine phosphatase activity assay of immunoprecipitated Shp1 on sorted ex vivo preselection thymocytes from Themis+/+ and Themis−/− mice with stimulation using biotinylated antibody cross-linking with streptavidin. (C) Titration of Themis by comparing Shp1 activity in unstimulated and stimulated thymocytes using biotinylated antibody cross-linking with streptavidin from Themis+/+, Themis+/−, and Themis−/− mice. (D) Tyrosine phosphatase activity assay of immunoprecipitated Shp1 on ex vivo sorted peripheral T cells from Themis+/+ and Themis−/− mice with and without stimulation using biotinylated antibody cross-linking with streptavidin. For sorting, two Themis+/+ and four Themis−/− mice were used. Data are representative of three experiments. Unpaired t test with mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. N.S., not significant.