Fig. 1.

Transcriptome profiling in the midgut by targeted DamID. (A) Schematic of midgut epithelial cell types. ISCs and EBs (green), ECs (red), and EEs (blue). ISCs divide to self-renew and generate differentiated EC and EE progeny via differentiating EB progenitors. (B and C) Example PolII DamID tracks (with unfused Dam control subtracted) for ISC/EB marker escargot and EC marker trypsin genes. (D) Genome-wide gene POLII occupancy in ISC/EBs vs. ECs with known markers highlighted (green for ISC/EB, red for EC). (E) Overlap in expressed genes in ISC/EB (green) vs. ECs (red) at FDR < 0.01.