(A–C) Markers for immune cells, including CD4, CD11b and CD45, were used to isolate subsets of T cells, macrophages and microglia by FACS from fresh brain and spinal cord 18 days after immunization. PTPσ−/− mice exhibit increased numbers of IFNγ+ and IL17+ CD4+ T cells, CD11b+CD45Hi macrophages and CD11b+CD45Int microglia monocytes. n = 3–5 mice/group.