Skip to main content
. Author manuscript; available in PMC: 2018 Dec 3.
Published in final edited form as: Brain Behav Immun. 2017 May 27;65:111–124. doi: 10.1016/j.bbi.2017.05.018

Figure 6. PTPσ deletion alters expression levels of cytokines and transcription factors in splenocytes 8 days after immunization.

Figure 6

(A) Schematic drawing shows the time points for experimental procedures. (B) Splenocytes were collected from WT and PTPσ−/− mice 8 days after MOG immunization (before disease onset) and re-stimulated with MOG in vitro for 3 days. Cytokine production was then evaluated by ELISA. (C, D) Splenocytes collected as in A were re-stimulated with MOG for 48 h, then qRT-PCR was used to measure levels of transcription factors that regulate CD4+ T cell differentiation and production of cytokine-encoded genes. * p<0.05, ** p<0.01 compared with WT mice. n = 3 mice/group.