Skip to main content
. 2018 Nov 20;3(11):15763–15770. doi: 10.1021/acsomega.8b02200

Figure 6.

Figure 6

Compound 12 inhibits fl-SHP2, but not fl-C333P SHP2. PTP activities of the indicated enzymes (250 nM) were measured with 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) (50 μM) in the absence (vehicle only) or presence of the indicated concentrations of compound 12 after a 2 h incubation at 37 °C (the reduced activity of fl-SHP2, as compared to the SHP2 PTP domain, necessitated a switch of substrate to DiFMUP, a small-molecule PTP substrate that is more efficient than pNPP).