TABLE II. Adult Nyssorhynchus nuneztovari s.l. collected in Lupuna (LUP), 2011-2012.
Month | Collection method | Total nº of Ny. nuneztovari s.l. collected (nº confirmed as Ny. nuneztovari s.l. by ITS2 PCR-RFLP) | Nº of Ny. nuneztovari s.l. confirmed as Ny. dunhami by COI sequence analysis |
February 2011 | Human landing catch: inside | 1 (0) | 0 |
Human landing catch: outside | 51 (21) | 20* | |
Shannon trap: outside | 147 (30) | 27 | |
April 2011 | CDC light trap: inside | 5 (4) | 4 |
Human landing catch: outside | 163 (87) | 60 | |
Shannon trap: outside | 202 (130) | 77 | |
June 2011 | Human landing catch: outside | 2 (0) | 0 |
October 2011 | Human landing catch: inside | 1 (0) | 0 |
Human landing catch: outside | 4 (0) | 0 | |
Shannon trap: outside | 1 (0) | 0 | |
December 2011 | Human landing catch: outside | 4 (0) | 0 |
Shannon trap: outside | 1 (0) | 0 | |
February 2012 | Human landing catch: inside | 1 (0) | 0 |
Human landing catch: outside | 3 (0) | 0 | |
June 2012 | Human landing catch: outside | 1 (1) | 0 |
Total | 587 (273) | 188 |
*: three infected with Plasmodium falciparum, one infected with P. vivax; COI: cytochrome c oxidase subunit I; PCR-RFLP: polymerase chain reaction-restriction fragment length polymorphism.