Figure 4.
Single-molecule imaging of the change of mAb-IR700 conjugates after NIR light irradiation. (a) 1.0 μm2 view of pan-IR700 on the mica was imaged with FM-AFM. After NIR light irradiation, more pan-IR700 is attached to the mica, and the size of pan-IR700 increases (4 J cm–2). (b) Single molecule of pan-IR700 on mica changes in volume and shape after NIR light irradiation. (c) Quantitative analysis of height and area of the particles on mica demonstrates that NIR-light-irradiated (4 J cm–2) pan-IR700 results in larger and longer molecules. Only pan-IR700 or irradiated panitumumab (pan) showed no change in the height and area (n = 3 to each group).