Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A Post-nuclear supernatant separation with OptiPrep density gradient centrifugation, followed by SDS-PAGE, in-gel protein digestion, and peptide analysis by LC–MS/MS. B Western blot analysis of TOMM20 in OptiPrep fractions of PNS collected with centrifugation speeds of 1000 and 5000 g. C Western blot analysis of proinsulin, STX6, and VAMP4 in each OptiPrep fraction. All antibodies were diluted 1:1000
