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. 2018 Aug 30;29(12):2456–2466. doi: 10.1007/s13361-018-2049-0

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Results of protein imaging of a complete liver tissue section. (a) 2D drift vs m/z plot (square root scale) with trend lines highlighted (HBA 1 and 2); red, haemoglobin subunit alpha-1/2 related; green, haemoglobin subunit beta-2 related (HBB2); blue, fatty acid binding protein (FABPL); yellow, 10 kDa heat shock protein (CH10); black, cytochrome c oxidase subunit 8A (COX8A). (b) (i) Extracted haemoglobin trend line; (ii) result of MaxEnt 1 algorithm deconvoluted spectrum i, showing haemoglobin related peaks. (c) Composite distributions for haemoglobin charge series. (d) Extracted trend line, MaxEnt 1 spectrum and combined ion image related to FABPL. (e) Extracted trend line, MaxEnt 1 spectrum and combined ion image related to CH10. (f) 4+ and 3+ charge states related and combined ion distribution image for COX8A (scale bar 4 mm)