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. 2018 Oct 29;8(19):5482–5500. doi: 10.7150/thno.28315

Figure 9.

Figure 9

The effect of different conditioned media (CMs) from polarized macrophages on osteogenic differentiation of BMSCs. (A) Images of ALP and AR staining and fluorescence immunolabeling of BMSCs after culture in CMs from M0, M1 and M2 macrophages when subjected to osteogenic supplements. Undiff: BMSCs cultured in standard medium only. Diff: osteogenic medium. Bar = 3 mm for entire plate views. Bar = 50 μm for fluorescence images. (B) Quantitative evaluation of ALP activity at day 7 and day 14 and quantification of the AR staining results at day 14. (C) Real-time qPCR analyses of ALP, COL1, OPN, RUNX2, BMP2 and OCN mRNA expression in BMSCs at day 7 and day 14. (D-E) ELISAs of CMs from M0, M1 and M2 macrophages to assess the levels of osteogenic (D) TGF-β1 and (E) BMP2 secretion. For all charts, the groups designated by different uppercase letters or lowercase letters were significantly different (p < 0.05).