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Preparation of mRNA-loaded polymeric nanoparticles. (A) Complexation of negatively charged FS-344 mRNA by positively charged PEG[pGlu(DET)]2 polymers into nanoparticles. (B) Modification of the mRNA-loaded nanoparticles by conjugation of PEG to polymer amino groups on the nanoparticle surface via amide bonds.
