Skip to main content
. 2018 Oct 22;8(19):5276–5288. doi: 10.7150/thno.27847

Figure 2.

Figure 2

(A) Viability of Huh7 human liver cells treated for 48 h with non- and PEG-modified mRNA nanoparticles prepared at the following N/P ratios: 0.5, 1, 2, 4, 6 and 8. Representative cryo-TEM image (B) and dynamic light scattering profile (C) of the PEG-modified, mRNA-loaded nanoparticles (N/P = 2). (D-E) Representative fluorescence microscopy images of Huh7 cells incubated for 48 h with nanoparticles loaded with the Cy5-labeled mRNA encoding for GFP. Images represent red fluorescence signal from Cy5-labeled mRNA (D) and green fluorescence signal generated by the expressed GFP (E). (F) Follistatin concentrations in the cell culture media quantified using ELISA after incubation of Huh7 and HEK293 human kidney cells for 48 h with and without nanoparticles containing FS-344 mRNA (250 ng/mL and 500 ng/mL). Results are not normalized for nanoparticle cell uptake differences. *p < 0.05 when compared with follistatin concentration produced by non-treated cells at the same time point.