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(A) Follistatin serum levels at various time points after a single subcutaneous injection of nanoparticles containing FS-344 mRNA (0.5 mg/kg). (B) Follistatin serum levels 24 h after the 16th injection. Mice were injected every 72 h for eight weeks with the nanoparticles (treatment) or saline (control). Serum concentrations of follistatin were analyzed using ELISA. *p < 0.05 when compared with the protein level in serum of non-treated animals.
