TABLE 4.
Association between selected gene variants and FR autoantibodies in NTDs and Controls
| IgGa |
IgMa |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SNP | N | Mean | SD | β | 95%CI | p | Mean | SD | β | 95%CI | p |
| NTD Cases(99) | |||||||||||
| rs1801133 (MTHFR) | |||||||||||
| Wildtype (CC) | 31 | 0.63 | 0.94 | Ref | 0.55 | 0.68 | Ref | ||||
| Heterozygous (CT) | 45 | 0.42 | 1.07 | −0.13 | −0.62, 0.49 | .592 | 0.45 | 0.92 | −.20 | −0.57, 0.18 | .312 |
| Homozygous (TT) | 23 | 0.74 | 0.85 | 0.16 | −0.43, 0.75 | .601 | 0.64 | 0.55 | −.04 | −0.50, 0.42 | .867 |
| rs1476413 (MTHFR) | |||||||||||
| Wildtype (AA) | 69 | 0.50 | 1.10 | Ref | 0.49 | 0.83 | Ref | ||||
| Heterozygous (AG) | 30 | 0.70 | 0.92 | 0.29 | −0.18, 0.77 | .228 | 0.61 | 0.98 | .26 | −0.11, 0.63 | .170 |
| rs7560488 (DNMT3A) | |||||||||||
| Wildtype (CC) | 62 | 0.43 | 1.01 | Ref | 0.57 | 0.74 | Ref | ||||
| Heterozygous (CT) | 32 | 0.71 | 0.94 | 0.35 | −0.10, 0.80 | .127 | 0.40 | 0.86 | −.13 | −0.48, 0.22 | .451 |
| Homozygous (TT) | 5 | 1.21 | 0.58 | 0.88 | 0.02, 1.75 | .044 | 0.76 | 0.54 | .21 | −0.46, 0.88 | .545 |
| rs828903 (MTHFD2) | |||||||||||
| Wildtype (AA) | 37 | 0.55 | 1.06 | Ref | 0.67 | 0.74 | Ref | ||||
| Heterozygous (AG) | 46 | 0.54 | 1.00 | −0.42 | −1.11, 0.28 | .238 | 0.34 | 0.86 | −.12 | −0.26, 0.19 | .384 |
| Homozygous (GG) | 16 | 0.66 | 0.77 | −0.68 | −2.03, 0.67 | .322 | 0.71 | 0.41 | −.47 | −0.51, 0.58 | .082 |
| rs7340453 (MTHFD2) | |||||||||||
| Wildtype (CC) | 39 | 0.46 | 1.11 | Ref | 0.54 | 0.95 | Ref | ||||
| Heterozygous (CT) | 41 | 0.58 | 0.97 | 0.38 | −0.31, 1.07 | .279 | 0.45 | 0.70 | .45 | −0.08,0.99 | .097 |
| Homozygous (TT) | 19 | 0.71 | 0.77 | 0.94 | −0.33, 2.21 | .146 | 0.66 | 0.44 | .51 | −0.47, 1.50 | .307 |
| rs70991108 (DHFR) | |||||||||||
| Wildtype (del/del) | 84 | 0.54 | 1.02 | Ref | 0.48 | 0.93 | Ref | ||||
| Heterozygous (del/ins) | 15 | 0.56 | 0.98 | −0.01 | −0.56, 0.53 | .959 | 0.53 | 0.74 | −.11 | −0.54, 0.31 | .590 |
| Controls (203) | |||||||||||
| rs1801133 (MTHFR) | |||||||||||
| Wildtype (CC) | 74 | −0.59 | 1.07 | Ref | −0.24 | 0.74 | Ref | ||||
| Heterozygous (CT) | 95 | −0.33 | 0.99 | 0.34 | 0.01, 0.67 | .044 | −0.003 | 0.76 | .27 | 0.03, 0.51 | .027 |
| Homozygous (TT) | 34 | −0.10 | 1.15 | 0.76 | 0.25, 1.28 | .004 | 0.23 | 0.75 | .54 | 0.17, 0.92 | .004 |
| rs1476413 (MTHFR) | |||||||||||
| Wildtype (AA) | 149 | −0.36 | 1.06 | Ref | −0.08 | 0.77 | Ref | ||||
| Heterozygous (AG) | 54 | −0.53 | 1.06 | −0.25 | −0.55, 0.86 | .267 | 0.01 | 0.75 | −.11 | −0.39, 0.18 | .469 |
| rs7560488 (DNMT3A) | |||||||||||
| Wildtype (CC) | 137 | −0.42 | 1.05 | Ref | −0.04 | 0.79 | Ref | ||||
| Heterozygous (CT) | 61 | −0.30 | 1.04 | 0.10 | −0.20, 0.41 | .505 | −0.07 | 0.73 | −.03 | −0.26, 0.19 | .765 |
| Homozygous (TT) | 5 | 0.11 | 1.11 | 0.48 | −0.44, 1.40 | .304 | 0.27 | 0.34 | .30 | −0.37, 0.97 | .376 |
| rs828903 (MTHFD2) | |||||||||||
| Wildtype (AA) | 84 | −0.58 | 0.95 | Ref | −0.16 | 0.67 | Ref | ||||
| Heterozygous (AG) | 99 | −0.27 | 1.13 | 0.09 | −0.32, 0.50 | .974 | −0.01 | 0.84 | .26 | −0.23, 0.37 | .649 |
| Homozygous (GG) | 20 | −0.01 | 0.93 | 0.51 | −0.21, 1.23 | .200 | 0.30 | 0.64 | .17 | 0.21, 1.26 | .006 |
| rs7340453 (MTHFD2) | |||||||||||
| Wildtype (CC) | 76 | −0.62 | 0.93 | Ref | −0.15 | 0.69 | Ref | ||||
| Heterozygous (CT) | 101 | −0.26 | 1.15 | 0.26 | −0.15, 0.68 | .209 | 0.02 | 0.81 | .08 | −0.22, 0.38 | .602 |
| Homozygous (TT) | 26 | −0.24 | 0.89 | 0.10 | −0.56, 0.77 | .761 | 0.004 | 0.77 | −.29 | −0.78, 0.19 | .235 |
| rs70991108 (DHFR) | |||||||||||
| Wildtype (del/del) | 168 | −0.33 | 1.01 | Ref | −0.05 | 0.66 | Ref | ||||
| Heterozygous (del/ins) | 35 | −0.41 | 1.06 | −0.01 | −0.39, 0.37 | .957 | −0.05 | 0.79 | −.07 | −0.35, 0.20 | .597 |
a
With adjustment for parity of women; IgG and IgM index were transformed using the natural logarithm