Table 3.
The primer sequences of target and internal reference genes used in qRT-PCR
| Gene | Forward primer | Reverse primer | PCR products (bp) |
|---|---|---|---|
| Erk1 | CTCAGCTTACGACCATGTGC | TCAGGTCCTGCACGATGTAG | 203 |
| Erk2 | CTCAGCAACGACCACATCTG | CCAGGCCAAAGTCACAGATC | 151 |
| p38 | ACAACATCGTCAAGTGCCAG | CACGTAGCCAGTCATCTCCT | 209 |
| JNK | TCAGTCAGTTGAGCACCAGT | ACTTATGCCTGCTCTGCTCA | 229 |
| Claudin-1 | CACCCTTGGCATGAAGTGTA | AGCCAATGAAGAGAGCCTGA | 216 |
| Claudin-4 | AAGGTGTACGACTCGCTGCT | GACGTTGTTAGCCGTCCAG | 238 |
| Occludin | GTTCGACCAATGCTCTCTCAG | CAGCTCCCATTAAGGTTCCA | 200 |
| ZO-1 | CGACCAGATCCTCAGGGTAA | AATCACCCACATCGGATTCT | 163 |
| GAPDH | GGGTCATCATCTCTGCACCT | GGTCATAAGTCCCTCCACGA | 180 |