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. 2018 Dec 3;18:316. doi: 10.1186/s12870-018-1556-2

Table 2.

Annotation, accession number and nucleotide sequences of primers to genes used for Real Time q-PCR

Gene annotation GenBank ID Forward primer 5′-3′ Reverse primer 5′-3′ Fragment length
BvActin HQ656028.1 ttgctgaccgtatgagcaag ttctgtggacgattgatgga 192
BvCYP76AD1 HQ656023 ttcacggccctttaatatcg tggcaagcatcaagtctttg 250
BvCYP76AD5 KM592961.1 gcgcatagacaatccaaggt gaatggggaagaaatcagca 241
BvCYP76AD6 KT962274 gctaaccgaaccattcctga tatcgacgggttgcattttt 223
BvDODA1 HQ656027 ggaaccagaattggcaagaa gagccaatgctcgtcctaag 209
BvMYB1 JF432080.1 atcgtcggcaaccataaaag atgcccacaagttcacaaca 248

Primers were designed using Primer3 online software