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. 2018 Nov 10;8(20):5676–5689. doi: 10.7150/thno.28538

Figure 2.

Figure 2

LncRNA DANCR promotes NPC cell migration and invasion in vitro. (A) Heatmap showing the genes altered (≥1.5-fold change) in SUNE-1 cells transfected with DANCR siRNA or scramble control, with 3 replicates. Gene expression is shown as RPKM after normalization. (B) KEGG pathway analysis for all dysregulated genes in SUNE-1 cells transfected with DANCR siRNA or scramble control. Cancer and metastasis-related terms were among the significant pathways for the genes dysregulated in the DANCR-silenced NPC cells. (C) Metastasis-related biological function enriched using gene set enrichment analysis (GSEA) in SUNE-1 cells transfected with DANCR siRNA or scramble control. P < 0.05, FDR ≤ 0.25. NES: normalized enrichment score. (D-E) Representative and quantification results of the wound healing (D) and Transwell migration and invasion assays (E) in HONE-1 and SUNE-1 cells transfected with DANCR siRNA or scramble control. (F-G) Representative and quantification results of the wound healing (F) and Transwell migration and invasion assays (G) in NP69 cells transfected with DANCR-overexpressing plasmid or vector. Scale bar, 100 μm. Data are presented as mean ± SD; Student's t-tests; *P<0.05. The experiments were independently repeated 3 times.