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Characterization of intermediates and dendrimer conjugates. A 1H NMR comparison of intermediates and final dendrimer conjugates showing the appearance/disappearance of characteristic proton signals; B HPLC traces of intermediates and final conjugates showing shifts in the retention time (6.6 minutes-bifunctional dendrimer; 7.8 minutes-free TPP; 7.3 minutes-TPP-D-Cy5 and 7.0 minutes-TPP-D-NAC); and C MALDI-ToF spectrum of TPP-D-NAC.
