Figure 6.

TPP-D-NAC enhances protection from oxidative stress induced cell death. Cells were pretreated for 24 hours with free NAC, D-NAC, or TPP-D-NAC, followed by oxidative stress insult of 500 µM H₂O₂ for 3 hours. A Cell viability was assessed with MTT assay. TPP-D-NAC exhibits significantly greater improvement in cell viability under oxidative stress compared to both DNAC and free NAC. *** p < 0.001, F = 33.706 with variable interaction in two-way ANOVA. B Cells were collected, treated with trypan blue, and counted in membrane exclusion assay. TPP-D-NAC exhibits significantly decreased cell death compared to D-NAC. ** p < 0.01, F = 9.569 with variable interaction in two-way ANOVA.