Figure 4.

PHB is essential for the MAPK signaling pathway activation and tumor growth induced by SLP2. A, B and C, Total cell lysates prepared from the indicated cells were subjected to immunoblot with different antibodies. D, 5000 indicated cells were plated in 96 culture-plates and OD values were measured. Data presented as means ± SEM from five independent experiments. ***p < 0.001, two-way ANOVA test. E, 500 indicated cells were plated in 6 well culture-plates for colony formation. Data presented as means ± SEM from three independent experiments. * p < 0.05, ** p < 0.01, ***p < 0.001, one-way ANOVA test. F, indicated cells (1×10⁶) were transplanted into NCG mice, and tumor growth was monitored. Data are presented as means ± SEM; n = 10 tumors for each group. ***p < 0.001, two-way ANOVA test. G and H, image and weights of tumors derived from hind limbs of NCG mice 60 days after subcutaneous injection of indicated cells (1×10⁶). Data are presented as the means ± SEM (n = 10). ***p < 0.001, Mann-Whitney test. I, representative H&E staining of primary tumors.